He mea make ka dihydrocoumarin

ʻO Dihydrocoumarin, mea ʻala, hoʻohana ʻia i ka meaʻai, hoʻohana ʻia hoʻi ma ke ʻano he coumarin pani, hoʻohana ʻia e like me ke ʻano hoʻonaninani; Hoʻohui i ka ʻaila, ka niu, ka ʻono kinamona; Hoʻohana ʻia ia ma ke ʻano he ʻono paka.

He mea make ka dihydrocoumarin

ʻAʻole ʻawaʻawa ʻo Dihydrocoumarin. ʻO Dihydrocoumarin kahi huahana kūlohelohe i loaʻa i ka rhinoceros vanilla melemele. Hoʻomākaukau ʻia e ka hydrogenation o coumarin i mua o ka nickel catalyst ma 160-200 ℃ a ma lalo o ke kaomi. Hiki ke hoʻohana ʻia e like me ka mea maka, hydrolyzed i loko o ka alkaline aqueous solution e hana i ka o-hydroxyphenylpropionic acid, dehydration, closed-loop i loaʻa.

Kūlana mālama

Paʻa a pōʻeleʻele, mālama ʻia i kahi maloʻo a maloʻo, ʻo ka wahi i loko o ka barela he liʻiliʻi loa ia ma lalo o nā ʻae palekana, a ua hoʻopiha ʻia me ka pale nitrogen. E kūʻai i loko o kahi hale kūʻai maʻalili. E noho mamao i ke ahi, ka wai. Pono e mālama kaʻawale mai ka oxidizer, mai hoʻohui i ka waihona. Hoʻolako ʻia me nā ʻano like ʻole a me ka nui o nā lako ahi.

Haʻawina in vitro

In vitro enzymatic assay, dihydrocoumarin induced concentration-dependent inhibition of SIRT1 (IC50 of 208μM). Ua ʻike ʻia ka emi ʻana o ka hana deacetylase SIRT1 a hiki i ka micromolar doses (85 ± 5.8 a me 73 ± 13.7% hana ma 1.6μM a me 8μM, kēlā me kēia). Ua kāohi pū ʻia ʻo Microtubule SIRT2 deacetylase i kahi ʻano like-dependence (IC50 o 295μM).

Ma hope o 24 mau hola o ka hoʻolaha ʻana, hoʻonui ka dihydrocoumarin (1-5mM) i ka cytotoxicity ma nā laina kelepona TK6 ma ke ʻano hilinaʻi. Ua hoʻonui ʻo Dihydrocoumarin (1-5mM) i ka apoptosis ma nā laina kelepona TK6 ma ke ʻano i hilinaʻi ʻia ma kahi manawa 6-hola. He 5mM ka nui o ka dihydrocoumarin i hoʻonui i ka apoptosis ma kahi manawa manawa 6-hola ma ka laina kelepona TK6. Ma hope o ka manawa hoʻolaha 24-hola, hoʻonui ka dihydrocoumarin (1-5mM) i ka p53 lysine 373 a me ka 382 acetylation ma ke ʻano o ka hopena i ka laina kelepona TK6.