ʻO Dihydrocoumarin, ʻala, hoʻohana ʻia i ka meaʻai, hoʻohana ʻia hoʻi ma ke ʻano he pani no ka coumarin, hoʻohana ʻia ma ke ʻano he mea ʻono hoʻonani; Hoʻohui ʻia ka kirīmi, ka niu, ka ʻono kinamona; Hoʻohana ʻia nō hoʻi ia ma ke ʻano he mea ʻono paka.
He mea ʻawahia anei ka dihydrocoumarin
ʻAʻole ʻawahia ka Dihydrocoumarin. He huahana kūlohelohe ka Dihydrocoumarin i loaʻa i loko o nā rhinoceros vanilla melemele. Hoʻomākaukau ʻia ia ma o ka hydrogenation o ka coumarin i ke alo o ka mea hoʻoulu nickel ma 160-200 ℃ a ma lalo o ke kaomi. Hiki ke hoʻohana ʻia ma ke ʻano he mea maka, hydrolyzed i loko o ka hopena wai alkaline e hana i ka waikawa o-hydroxyphenylpropionic, dehydration, loaʻa ka loop pani.
Kūlana mālama
Pani ʻia a pouli, mālama ʻia ma kahi anu a maloʻo, ʻo ka hakahaka i loko o ka pahu e like me ka liʻiliʻi ma lalo o nā ʻae palekana, a ua piha i ka pale naikokene. E mālama i loko o kahi hale kūʻai anu a me ka ea. E noho mamao mai ke ahi a me ka wai. Pono e mālama ʻokoʻa mai ka oxidizer, mai hoʻohuihui i ka waihona. Hoʻolako ʻia me nā ʻano like ʻole a me ka nui o nā lako ahi.
Haʻawina in vitro
Ma ka hoʻāʻo enzymatic in vitro, ua hoʻoulu ka dihydrocoumarin i ka pale ʻana o ka SIRT1 (IC50 o 208μM). Ua ʻike ʻia ka emi ʻana o ka hana SIRT1 deacetylase ʻoiai ma nā dosis micromolar (85±5.8 a me 73±13.7% hana ma 1.6μM a me 8μM, kēlā me kēia). Ua pale ʻia hoʻi ka Microtubule SIRT2 deacetylase ma ke ʻano like e pili ana i ka dosis (IC50 o 295μM).
Ma hope o 24 mau hola o ka hōʻike ʻana, ua hoʻonui ka dihydrocoumarin (1-5mM) i ka cytotoxicity i nā laina cell TK6 ma ke ʻano hilinaʻi i ka lāʻau. Ua hoʻonui ka Dihydrocoumarin (1-5mM) i ka apoptosis i nā laina cell TK6 ma ke ʻano hilinaʻi i ka lāʻau i kahi manawa 6 hola. Ua hoʻonui ka lāʻau 5mM o ka dihydrocoumarin i ka apoptosis i kahi manawa 6 hola ma ka laina cell TK6. Ma hope o kahi manawa hōʻike 24 hola, ua hoʻonui ka dihydrocoumarin (1-5mM) i ka p53 lysine 373 a me 382 acetylation ma ke ʻano hilinaʻi i ka lāʻau ma ka laina cell TK6.
Ka manawa hoʻouna: Nov-01-2024